Article
Transport of the yeast ATP synthase beta-subunit into mitochondria. Effects of amino acid substitutions on targeting.
The Biochemical journal - 15 Feb 1990
Walker M E, Valentin E, Reid G A
Abstract excerpt
We have isolated the yeast ATP2 gene encoding the beta-subunit of mitochondrial ATP synthase and determined its nucleotide sequence. A fusion between the N-terminal 15 amino acid residues of beta-subunit and the mouse cytosolic protein dihydrofolate reductase (DHFR) was transcribed and translated...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Escherichia coli
- Mitochondria
- Molecular Sequence Data
- Mutation
- Protein Precursors
- Proton-Translocating ATPases
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Structure-Activity Relationship
- Tetrahydrofolate Dehydrogenase
- Transformation, Genetic