Article
Transport of the yeast ATP synthase beta-subunit into mitochondria. Effects of amino acid substitutions on targeting.
The Biochemical journal - 15 Feb 1990
Walker M E, Valentin E, Reid G A
Abstract excerpt
We have isolated the yeast ATP2 gene encoding the beta-subunit of mitochondrial ATP synthase and determined its nucleotide sequence. A fusion between the N-terminal 15 amino acid residues of beta-subunit and the mouse cytosolic protein dihydrofolate reductase (DHFR) was transcribed and translated in vitro and found to be transported into isolated yeast mitochondria. A fusion with the first 35 amino acid residues...
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