Article
High level expression and characterization of the mitochondrial citrate transport protein from the yeast Saccharomyces cerevisiae.
The Journal of biological chemistry - 24 Feb 1995
Kaplan R S, Mayor J A, Gremse D A, Wood D O
Abstract excerpt
The gene encoding the mitochondrial citrate transport protein (CTP) in the yeast Saccharomyces cerevisiae has been identified, and its protein product has been overexpressed in Escherichia coli. The expressed CTP accumulates in inclusion bodies and can be solubilized with sarkosyl. Approximately 25 mg of solubilized CTP at a purity of 75% is obtained per liter of E. coli culture. The function of the solubilized...
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