Article
ATP synthase of yeast mitochondria. Isolation of the F1 delta subunit, sequence and disruption of the structural gene.
European journal of biochemistry - 15 Jun 1994
Giraud M F, Velours J
Abstract excerpt
The delta-subunit was isolated from the purified yeast F1. Partial protein sequences were determined by direct methods. From this information, degenerated primers were constructed. A part of the ATP delta gene was amplified by polymerase chain reaction from yeast genomic DNA. From the amplified D...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Gene Amplification
- Genes, Fungal
- Mitochondria
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Neurospora crassa
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Proton-Translocating ATPases
- Saccharomyces cerevisiae
- Sequence Alignment