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Article

ATP synthase of yeast mitochondria. Isolation of the F1 delta subunit, sequence and disruption of the structural gene.

European journal of biochemistry - 15 Jun 1994

Giraud M F, Velours J

Abstract excerpt

The delta-subunit was isolated from the purified yeast F1. Partial protein sequences were determined by direct methods. From this information, degenerated primers were constructed. A part of the ATP delta gene was amplified by polymerase chain reaction from yeast genomic DNA. From the amplified DNA sequence, a nondegenerated oligonucleotide probe was constructed to isolate a 2.6-kbp BamHI-EcoRI DNA fragment...

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Source

Identifiers

PMID: 8026496
DOI: 10.1111/j.1432-1033.1994.tb18932.x

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