Article
Transforming activity of a novel mutant of HPV16 E6E7 fusion gene.
Virologica Sinica - 1 Jun 2011
Xie Qiang, Zhou Zhi-Xiang, Li Ze-Lin, Zeng Yi
Abstract excerpt
An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost...
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