Article
Intracellular protein labeling with prodrug-like probes using a mutant β-lactamase tag.
Chemistry (Weinheim an der Bergstrasse, Germany) - 18 Jul 2011
Watanabe Shuji, Mizukami Shin, Akimoto Yuri, Hori Yuichiro, Kikuchi Kazuya
Abstract excerpt
Intracellular protein labeling with small molecular probes that do not require a washing step for the removal of excess probe is greatly desired for real-time investigation of protein dynamics in living cells. Successful labeling of proteins on the cell membrane has been performed using mutant β-lactamase tag (BL-tag) technology. In the present study, intracellular protein labeling with novel cell membrane...
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