Article
Monitoring endocytic trafficking of anthrax lethal factor by precise and quantitative protein labeling.
Angewandte Chemie (International ed. in English) - 16 Jun 2014
Zheng Siqi, Zhang Gong, Li Jie, Chen Peng R
Abstract excerpt
Coupling the genetic code expansion technique with bioorthogonal reactions enables precise control over the conjugation site as well as the choice of fluorescent probes during protein labeling. However, the advantages of this strategy over bulky and rigid fluorescent proteins (FPs) remain to be fully explored. Here we applied site-specific bioorthogonal labeling on anthrax lethal factor (LF) to visualize its...
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