Article
A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments.
Biotechnology journal - 1 Mar 2014
Kim Edward Y, Tullman-Ercek Danielle
Abstract excerpt
Bacterial microcompartments (MCPs) are protein-based organelles that have been suggested as scaffolds for creating in vivo nanobioreactors. One of the key steps towards engineering MCPs is to understand and maximize the encapsulation of enzymes into the lumen of the MCP. However, there are currently no high-throughput methods for investigating protein encapsulation. Here, we describe the development of a rapid in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
