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Article

Biochemical characterization of baculovirus-expressed rap1A/Krev-1 and its regulation by GTPase-activating proteins.

Molecular and cellular biology - 1 Jun 1990

Quilliam L A, Der C J, Clark R, O'Rourke E C, Zhang K, McCormick F, Bokoch G M

Abstract excerpt

Normal human rap1A and 35A rap1A (which encodes a protein with a Thr-35----Ala mutation) were cloned into a baculovirus transfer vector and expressed in Sf9 insect cells. The resulting proteins were purified, and their nucleotide binding, GTPase activities, and responsiveness to GTPase-activating proteins (GAPs) were characterized and compared with those of Rap1 purified from human neutrophils. Recombinant...

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Identifiers

PMID: 2160589
DOI: 10.1128/mcb.10.6.2901-2908.1990
PMCID: PMC360652

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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