Article
Biochemical characterization of baculovirus-expressed rap1A/Krev-1 and its regulation by GTPase-activating proteins.
Molecular and cellular biology - 1 Jun 1990
Quilliam L A, Der C J, Clark R, O'Rourke E C, Zhang K, McCormick F, Bokoch G M
Abstract excerpt
Normal human rap1A and 35A rap1A (which encodes a protein with a Thr-35----Ala mutation) were cloned into a baculovirus transfer vector and expressed in Sf9 insect cells. The resulting proteins were purified, and their nucleotide binding, GTPase activities, and responsiveness to GTPase-activating...
Topics
- Animals
- Base Sequence
- Cell Line
- Cell Membrane
- Cloning, Molecular
- GTP Phosphohydrolases
- GTP-Binding Proteins
- GTPase-Activating Proteins
- Gene Expression Regulation
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Diphosphate
- Guanosine Triphosphate
- Humans
- Insect Viruses