Article
RA-GEF-1, a guanine nucleotide exchange factor for Rap1, is activated by translocation induced by association with Rap1*GTP and enhances Rap1-dependent B-Raf activation.
The Journal of biological chemistry - 27 Jul 2001
Liao Y, Satoh T, Gao X, Jin T G, Hu C D, Kataoka T
Abstract excerpt
We previously identified RA-GEF-1, a novel guanine nucleotide exchange factor (GEF) for Rap1 with the ability to associate with Rap1.GTP at its Ras/Rap1-associating (RA) domain. Because it possesses a PSD-95/DlgA/ZO-1 (PDZ) domain, it was also named PDZ-GEF. In this report, we have examined the role of the RA domain of this protein in Rap1-mediated cellular responses. A mutant of RA-GEF-1 (RA-GEF-1DeltaRA)...
Topics
- Animals
- COS Cells
- Cell Nucleus
- Cyclic AMP
- Cyclic GMP
- Enzyme Activation
- Gene Deletion
- Guanine Nucleotide Exchange Factors
- Microscopy, Fluorescence
- Mutagenesis, Site-Directed
- Mutation
- Nerve Tissue Proteins
- Plasmids