Article
Saturation mutagenesis of a DNA region of bend. Base steps other than ApA influence the bend.
Journal of molecular biology - 5 May 1990
Milton D L, Casper M L, Gesteland R F
Abstract excerpt
A 60 base-pair region of a simian virus 40 DNA fragment was mutagenized to determine base-pairs that are critical for the fragment to bend. The site-directed mutagenesis saturated this region with all possible single base-pair substitutions. The mobility of each mutated fragment was measured by polyacrylamide electrophoresis at 4 degrees C and at 65 degrees C to assess the degree of bend. Four conclusions can be...
Topics
- Base Composition
- Base Sequence
- Capsid
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Simian virus 40