Article
Sequence-dependent enhancement of hydrolytic deamination of cytosines in DNA by the restriction enzyme PspGI.
Nucleic acids research - 1 Jan 2006
Carpenter Michael, Divvela Pradeep, Pingoud Vera, Bujnicki Janusz, Bhagwat Ashok S
Abstract excerpt
Hydrolytic deamination of cytosines in DNA creates uracil and, if unrepaired, these lesions result in C to T mutations. We have suggested previously that a possible way in which cells may prevent or reduce this chemical reaction is through the binding of proteins to DNA. We use a genetic reversion assay to show that a restriction enzyme, PspGI, protects cytosines within its cognate site, 5'-CCWGG (W is A or T),...
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