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Article

High level expression of wild type and variant mouse glucocorticoid receptors in Chinese hamster ovary cells.

Molecular endocrinology (Baltimore, Md.) - 1 Jan 1990

Hirst M A, Northrop J P, Danielsen M, Ringold G M

Abstract excerpt

We have isolated Chinese hamster ovary (CHO) cell lines expressing elevated levels of wild-type (W) and mutant forms of the glucocorticoid receptor (GR) using the technique of coamplification with a selectable dihydrofolate reductase (dhfr) cDNA. A prominent doublet at 90/92 kilodaltons was observed by Western blotting or labeling with [3H]-dexamethasone mesylate in extracts from cells transfected with W, the...

Topics

Animals
Cell Line
Cricetinae
Cricetulus
DNA
Dexamethasone
Gene Amplification
Gene Expression
Genetic Variation
Mammary Tumor Virus, Mouse
Mice
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Glucocorticoid

Tetrahydrofolate Dehydrogenase

Transfection

Source

Identifiers

PMID: 2157974
DOI: 10.1210/mend-4-1-162

Genes

CAT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2157974-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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