Article
Biochemical evidence that glucocorticoid-sensitive cell lines derived from the human leukemic cell line CCRF-CEM express a normal and a mutant glucocorticoid receptor gene.
Cancer research - 1 Oct 1991
Palmer L A, Harmon J M
Abstract excerpt
To characterize the immunoreactive glucocorticoid receptor (GR) protein present in "receptorless" (r-) mutants isolated from the glucocorticoid-sensitive (dexs) human leukemic cell line CEM-C7, binding of [3H]dexamethasone was determined in extracts prepared from the sensitive cell line 6TG1.1 and the r- mutant ICR27TK.3 after gentle freeze-thaw lysis and low-speed centrifugation. Under these conditions there was...
Topics
- Binding, Competitive
- Blotting, Western
- Cell Line
- Chromatography, DEAE-Cellulose
- Dexamethasone
- Humans
- Leukemia
- Molybdenum
- Mutation
- Polymorphism, Genetic
- Receptors, Glucocorticoid