Article
Identification of the activation-labile gene: a single point mutation in the human glucocorticoid receptor presents as two distinct receptor phenotypes.
Molecular endocrinology (Baltimore, Md.) - 1 May 1993
Ashraf J, Thompson E B
Abstract excerpt
CCRF-CEM-C7 is a well characterized human leukemic clonal cell line which is lysed by dexamethasone (dex). Originating from the wild-type CEM-C7 cells are two dex-resistant clones which are not lysed by 1 microM dex and have functionally defective glucocorticoid receptors (GR). They are receptorl...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Dexamethasone
- Drug Resistance
- Humans
- Leukemia
- Molecular Sequence Data
- Phenotype
- Plasmids
- Point Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- Receptors, Glucocorticoid
- Sequence Analysis, DNA
- Transfection
- Tumor Cells, Cultured