Article
Functional importance of telomerase pseudoknot revealed by single-molecule analysis.
Proceedings of the National Academy of Sciences of the United States of America - 20 Dec 2011
Mihalusova Mariana, Wu John Y, Zhuang Xiaowei
Abstract excerpt
Telomerase ribonucleoprotein (RNP) employs an RNA subunit to template the addition of telomeric repeats onto chromosome ends. Previous studies have suggested that a region of the RNA downstream of the template may be important for telomerase activity and that the region could fold into a pseudoknot. Whether the pseudoknot motif is formed in the active telomerase RNP and what its functional role is have not yet...
Topics
- Base Sequence
- Biotinylation
- Catalysis
- Fluorescence Resonance Energy Transfer
- Holoenzymes
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA
- Telomerase
- Tetrahymena thermophila