Non-phenotypic selection of N-methyl-N'-nitro-N-nitrosoguanidine-directed mutation at a predicted hotspot site.

doi:10.1016/0165-7992(90)90037-k

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Article

Non-phenotypic selection of N-methyl-N'-nitro-N-nitrosoguanidine-directed mutation at a predicted hotspot site.

Mutation research - 1 Feb 1990

Gordon A J, Schy W E, Glickman B W

Abstract excerpt

The striking mutational specificity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) exhibited in the lacI gene in Escherichia coli allows comment on the phenotypic consequences of mutation at specific sequences that are not recovered after MNNG mutagenesis. We predict that the I+ phenotype is main...

Topics

Bacterial Proteins
Base Sequence
Codon
DNA Mutational Analysis
DNA, Bacterial
Escherichia coli
Glycine
Methylnitronitrosoguanidine
Molecular Sequence Data
Mutation
Phenotype
Repressor Proteins

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