Article
Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus.
Science (New York, N.Y.) - 13 May 2011
Watanabe Toshiaki, Tomizawa Shin-ichi, Mitsuya Kohzoh, Totoki Yasushi, Yamamoto Yasuhiro, Kuramochi-Miyagawa Satomi, Iida Naoko, Hoki Yuko, Murphy Patrick J, Toyoda Atsushi, Gotoh Kengo, Hiura Hitoshi, Arima Takahiro, Fujiyama Asao, Sado Takashi, Shibata Tatsuhiro, Nakano Toru, Lin Haifan, Ichiyanagi Kenji, Soloway Paul D, Sasaki Hiroyuki
Abstract excerpt
Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated...
Topics
- Animals
- Argonaute Proteins
- DNA Methylation
- Genomic Imprinting
- Male
- Mice
- Mice, Inbred C57BL
- Mitochondrial Proteins
- Models, Genetic