Article
Establishment and characterization of a new Epstein-Barr virus transformed cell line from a human B cell lymphoma.
Blut - 1 Mar 1990
Janssen O, Heinze B, Bartram C R, Pawlita M, Binder T, Heimpel H, Diehl K, Kabelitz D
Abstract excerpt
We have established a new cell line from a patient with centrocytic B cell lymphoma. Highly purified peripheral blood B cells from patient DUL (WBC counts 158,000/microliters) were infected in vitro with Epstein-Barr virus (EBV), and CD20+ B cells were cloned into 96 well culture plates with the aid of a cell sorter autoclone device. As shown by GTG-banding and Southern blot analysis, out-growing EBV-positive...
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