Article
Establishment and characterization of Epstein-Barr virus-specific human CD4+ T lymphocyte clones.
Acta virologica - 1 Nov 1998
Honda S, Takasaki T, Okuno K, Yasutomi M, Kurane I
Abstract excerpt
We developed a simple method for establishing Epstein-Barr virus (EBV)-specific, human CD4+ T cell clones. The method originates from our experience that the regression of cell growth in in vitro EBV transformation of B cells occurs when round lymphoid cells appear in the culture. Peripheral blood mononuclear cells (PBMCs) were cultured with EBV, and IL-2 (20 U/ml) was added to the culture on day 17 after the...
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