Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Saturation mutagenesis of the DNA site bound by the small carboxy-terminal domain of gamma delta resolvase.

The EMBO journal - 1 Mar 1990

Rimphanitchayakit V, Grindley N D

Abstract excerpt

We have analyzed the sequence requirements for the binding of the carboxy-terminal (DNA binding) domain of gamma delta resolvase to its recognition site. Using an efficient procedure for saturation mutagenesis we have obtained 31 of the possible 36 base substitutions within the 12 bp minimal bind...

Topics

Base Composition
Base Sequence
Binding Sites
Cloning, Molecular
DNA
Molecular Sequence Data
Mutation
Nucleotidyltransferases
Plasmids
Restriction Mapping
Substrate Specificity
Transposases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 2155779
DOI: 10.1002/j.1460-2075.1990.tb08165.x
PMCID: PMC551726

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2155779-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 10 closest

Saturation mutagenesis of the DNA site bound by the small carboxy-terminal domain of gamma delta resolvase.PMID 2155779Read ↗

Secondary and tertiary structural changes in gamma delta resolvase: comparison of the wild-type enzyme, the I110R mutant, and the C-terminal DNA binding domain in solution.Semantic proximity Read ↗

Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.Semantic proximity Read ↗

The tyrosine-6 hydroxyl of gamma delta resolvase is not required for the DNA cleavage and rejoining reactions.Semantic proximity Read ↗

Refined solution structure of the C-terminal DNA-binding domain of human immunovirus-1 integrase.Semantic proximity Read ↗

Mutational analysis of a site-specific recombinase: characterization of the catalytic and dimerization domains of the beta recombinase of pSM19035.Semantic proximity Read ↗

Two basic residues, Lys-107 and Lys-118, of RuvC resolvase are involved in critical contacts with the Holliday junction for its resolution.Semantic proximity Read ↗

Cooperative binding of Tn3 resolvase monomers to a functionally asymmetric binding site.Semantic proximity Read ↗

Genetic analysis of the base-specific contacts of BamHI restriction endonuclease.Semantic proximity Read ↗

Effect of non-contacted bases on the affinity of 434 operator for 434 repressor and Cro.Semantic proximity Read ↗

Structure of a synaptic gammadelta resolvase tetramer covalently linked to two cleaved DNAs.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.