Article
Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.
Nature genetics - 1 Feb 1995
Mashal R D, Koontz J, Sklar J
Abstract excerpt
We have explored the application of the bacteriophage resolvases T4 endonuclease VII and T7 endonuclease I for detecting mutations in genomic DNA. Heteroduplex DNA fragments prepared by amplification from DNA containing known mutations were cleaved by one or both enzymes at nucleotide mismatches...
Topics
- Bacteriophages
- Base Sequence
- DNA
- DNA Mutational Analysis
- Deoxyribonuclease I
- Endodeoxyribonucleases
- Humans
- Methods
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes