Article
Mutational analysis of a site-specific recombinase: characterization of the catalytic and dimerization domains of the beta recombinase of pSM19035.
Molecular & general genetics : MGG - 1 Aug 1997
Canosa I, Ayora S, Rojo F, Alonso J C
Abstract excerpt
The beta recombinase encoded by the streptococcal plasmid pSM19035, which shows 28 to 34% identity with DNA resolvases and DNA invertases, can catalyze formation of deletions or inversions between properly oriented target sites. We have constructed a number of site-directed mutations at residues...
Topics
- Amino Acid Sequence
- Binding Sites
- DNA Nucleotidyltransferases
- DNA, Bacterial
- Dimerization
- Escherichia coli
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Recombination, Genetic
- Sequence Homology, Amino Acid
- Streptococcus