Article
Site-specific mutagenesis of a highly conserved region of the herpes simplex virus type 1 DNA polymerase gene.
Journal of virology - 1 Mar 1990
Dorsky D I, Crumpacker C S
Abstract excerpt
Seven point mutations were introduced into region I of the herpes simplex virus type 1 DNA polymerase, which is most highly conserved among DNA polymerases and has no drug sensitivity markers mapped to it. The functional consequences of these mutations were studied in an in vitro transcription-tr...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA-Directed DNA Polymerase
- Genes, Viral
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Protein Biosynthesis
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Simplexvirus
- T-Phages
- Viral Structural Proteins