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Article

Site-specific mutagenesis of a highly conserved region of the herpes simplex virus type 1 DNA polymerase gene.

Journal of virology - 1 Mar 1990

Dorsky D I, Crumpacker C S

Abstract excerpt

Seven point mutations were introduced into region I of the herpes simplex virus type 1 DNA polymerase, which is most highly conserved among DNA polymerases and has no drug sensitivity markers mapped to it. The functional consequences of these mutations were studied in an in vitro transcription-translation system in which T7 transcripts of cloned polymerase genes were used to generate enzymatically active...

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Identifiers

PMID: 2154619
DOI: 10.1128/JVI.64.3.1394-1397.1990
PMCID: PMC249264

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