Isolation and characterization of herpes simplex virus mutants containing engineered mutations at the DNA polymerase locus.

doi:10.1128/JVI.64.5.2208-2216.1990

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Article

Isolation and characterization of herpes simplex virus mutants containing engineered mutations at the DNA polymerase locus.

Journal of virology - 1 May 1990

Marcy A I, Yager D R, Coen D M

Abstract excerpt

We have derived Vero cell lines containing the herpes simplex virus DNA polymerase (pol) gene that complement temperature-sensitive pol mutants. These cell lines were used to recover viruses containing new mutations at the pol locus. Two spontaneously arising host-range mutants, 6C4 and 7E4, were isolated. These mutants did not grow efficiently on Vero cells or synthesize late polypeptides but formed plaques on a...

Topics

Animals
Base Sequence
Blotting, Southern
Chromosome Deletion
DNA-Directed DNA Polymerase
Escherichia coli
Genes, Bacterial
Genes, Viral
Genetic Complementation Test
Genetic Engineering
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oligonucleotide Probes

Plasmids

Restriction Mapping

Simplexvirus

Transcription, Genetic

Vero Cells

Source

Open in PubMed Open full text

Identifiers

PMID: 2157881
DOI: 10.1128/JVI.64.5.2208-2216.1990
PMCID: PMC249381

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2157881-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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