Article
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.
Cell - 29 Apr 2011
Green Rebecca A, Kao Huey-Ling, Audhya Anjon, Arur Swathi, Mayers Jonathan R, Fridolfsson Heidi N, Schulman Monty, Schloissnig Siegfried, Niessen Sherry, Laband Kimberley, Wang Shaohe, Starr Daniel A, Hyman Anthony A, Schedl Tim, Desai Arshad, Piano Fabio, Gunsalus Kristin C, Oegema Karen
Abstract excerpt
High-content screening for gene profiling has generally been limited to single cells. Here, we explore an alternative approach-profiling gene function by analyzing effects of gene knockdowns on the architecture of a complex tissue in a multicellular organism. We profile 554 essential C. elegans genes by imaging gonad architecture and scoring 94 phenotypic features. To generate a reference for evaluating methods...
Topics
- Animals
- Caenorhabditis elegans
- Computational Biology
- Embryo, Nonmammalian
- Gene Knockdown Techniques
- Gene Regulatory Networks
- Genetic Techniques
- Gonads
- Phenotype