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Article

Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics.

Biomolecules - 20 Jul 2015

Soga Keisuke, Abo Hirohito, Qin Sheng-Ying, Kyoutou Takuya, Hiemori Keiko, Tateno Hiroaki, Matsumoto Naoki, Hirabayashi Jun, Yamamoto Kazuo

Abstract excerpt

Leguminous lectins have a conserved carbohydrate recognition site comprising four loops (A-D). Here, we randomly mutated the sequence and length of loops C and D of peanut agglutinin (PNA) and expressed the proteins on the surface of mouse green fluorescent protein (GFP)-reporter cells. Flow cytometry, limiting dilution, and cDNA cloning were used to screen for several mutated PNAs with distinct properties. The...

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Identifiers

PMID: 26287256
DOI: 10.3390/biom5031540
PMCID: PMC4598763

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Source receipt: pubmed, retrieved 7 Sept 2026.

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