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Article

Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors.

Chemistry & biology - 22 Apr 2011

Ito Tamaki, Umehara Takashi, Sasaki Kazuki, Nakamura Yoshihiro, Nishino Norikazu, Terada Takaho, Shirouzu Mikako, Padmanabhan Balasundaram, Yokoyama Shigeyuki, Ito Akihiro, Yoshida Minoru

Abstract excerpt

Histone acetylation constitutes an epigenetic mark for transcriptional regulation. Here we developed a fluorescent probe to visualize acetylation of histone H4 Lys12 (H4K12) in living cells using fluorescence resonance energy transfer (FRET) and the binding of the BRD2 bromodomain to acetylated H4K12. Using this probe designated as Histac-K12, we demonstrated that histone H4K12 acetylation is retained in mitosis...

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Source

Identifiers

PMID: 21513886
DOI: 10.1016/j.chembiol.2011.02.009

Genes

BRD2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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