Article
An essential proline in lambda repressor is required for resistance to intracellular proteolysis.
Biochemistry - 21 Aug 1990
Reidhaar-Olson J F, Parsell D A, Sauer R T
Abstract excerpt
Pro78 is a solvent-exposed residue at the N-terminal end of alpha-helix 5 in the DNA binding domain of lambda repressor. Random mutagenesis experiments have suggested that Pro78 is essential [Reidhaar-Olson, J.F., & Sauer, R.T. (1990) Proteins: Struct., Funct., Genet. (in press)]. To investigate the requirement for proline at this position, we constructed and studied the properties of a set of ten position 78...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- DNA, Bacterial
- Endopeptidases
- Escherichia coli
- Hot Temperature
- Molecular Sequence Data
- Mutation
- Proline
- Protein Denaturation
- Repressor Proteins