Article
Correction of PCR-bias in quantitative DNA methylation studies by means of cubic polynomial regression.
Nucleic acids research - 1 Jun 2011
Moskalev Evgeny A, Zavgorodnij Mikhail G, Majorova Svetlana P, Vorobjev Ivan A, Jandaghi Pouria, Bure Irina V, Hoheisel Jörg D
Abstract excerpt
DNA methylation profiling has become an important aspect of biomedical molecular analysis. Polymerase chain reaction (PCR) amplification of bisulphite-treated DNA is a processing step that is common to many currently used methods of quantitative methylation analysis. Preferential amplification of unmethylated alleles-known as PCR-bias-may significantly affect the accuracy of quantification. To date, no universal...
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