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Isometric artifacts from polymerase chain reaction-massively parallel sequencing analysis of short tandem repeat loci: An emerging issue from a new technology?

Electrophoresis - 1 Jul 2022

Zupanič Pajnič Irena, Previderè Carlo, Zupanc Tomaž, Zanon Martina, Fattorini Paolo

Abstract excerpt

The recent introduction of polymerase chain reaction (PCR)-massively parallel sequencing (MPS) technologies in forensics has changed the approach to allelic short tandem repeat (STR) typing because sequencing cloned PCR fragments enables alleles with identical molecular weights to be distinguished based on their nucleotide sequences. Therefore, because PCR fidelity mainly depends on template integrity, new...

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PMID: 35358339
DOI: 10.1002/elps.202100143
PMCID: PMC9543752

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