Article
Investigation of Linear Amplification Using Abasic Site-Containing Primers Coupled to Routine STR Typing for LT-DNA Analysis.
Genes - 4 Aug 2022
Qian Xiaoqin, Li Zhimin, Zhou Zhihan, Qian Jinglei, Yao Yining, Shao Chengchen, Tang Qiqun, Xie Jianhui
Abstract excerpt
Obtaining a full short tandem repeat (STR) profile from a low template DNA (LT-DNA) still presents a challenge for conventional methods due to significant stochastic effects and polymerase slippage. A novel amplification method with a lower cost and higher accuracy is required to improve the DNA amount. Previous studies suggested that DNA polymerases without bypass activity could not perform processive DNA...
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