Article
A survey of new temperature-sensitive, embryonic-lethal mutations in C. elegans: 24 alleles of thirteen genes.
PloS one - 1 Mar 2011
O'Rourke Sean M, Carter Clayton, Carter Luke, Christensen Sara N, Jones Minh P, Nash Bruce, Price Meredith H, Turnbull Douglas W, Garner Aleena R, Hamill Danielle R, Osterberg Valerie R, Lyczak Rebecca, Madison Erin E, Nguyen Michael H, Sandberg Nathan A, Sedghi Noushin, Willis John H, Yochem John, Johnson Eric A, Bowerman Bruce
Abstract excerpt
To study essential maternal gene requirements in the early C. elegans embryo, we have screened for temperature-sensitive, embryonic lethal mutations in an effort to bypass essential zygotic requirements for such genes during larval and adult germline development. With conditional alleles, multiple essential requirements can be examined by shifting at different times from the permissive temperature of 15°C to the...
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