Preparation, application and biological characterization of interleukin-1 beta mutant protein biotinylated at a single site.

doi:10.1016/0022-1759(90)90067-6

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Article

Preparation, application and biological characterization of interleukin-1 beta mutant protein biotinylated at a single site.

Journal of immunological methods - 9 Mar 1990

Chollet A, Bonnefoy J Y, Odermatt N

Abstract excerpt

Selective biotinylation of human interleukin-1 beta was achieved by reaction of an interleukin-1 beta mutant protein containing a surface-accessible cysteine with maleimido-biotin. A defined interleukin-1 beta derivative biotinylated at a single site was obtained. This compound retained full affi...

Topics

Animals
Bacterial Proteins
Biotin
Flow Cytometry
Humans
Interleukin-1
Mice
Mutation
Receptors, Immunologic
Receptors, Interleukin-1
Streptavidin

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