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Article

Preparation, application and biological characterization of interleukin-1 beta mutant protein biotinylated at a single site.

Journal of immunological methods - 9 Mar 1990

Chollet A, Bonnefoy J Y, Odermatt N

Abstract excerpt

Selective biotinylation of human interleukin-1 beta was achieved by reaction of an interleukin-1 beta mutant protein containing a surface-accessible cysteine with maleimido-biotin. A defined interleukin-1 beta derivative biotinylated at a single site was obtained. This compound retained full affinity for both the interleukin-1 receptor and streptavidin or avidin; however, its biological activity was significantly...

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Identifiers

PMID: 2138199
DOI: 10.1016/0022-1759(90)90067-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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