Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.

Abstract excerpt

For a multistep pre-targeting method using antibodies, a streptavidin mutant with low immunogenicity, termed low immunogenic streptavidin mutant No. 314 (LISA-314), was produced previously as a drug delivery tool. However, endogenous biotins (BTNs) with high affinity (Kd < 10(-10) M) for the binding pocket of LISA-314 prevents access of exogenous BTN-labelled anticancer drugs. In this study, we improve the...

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• Biotin
• Crystallography, X-Ray
• Models, Molecular
• Mutation
• Protein Conformation