Article
TEM-1 beta-lactamase as a scaffold for protein recognition and assay.
Protein science : a publication of the Protein Society - 1 Jun 2002
Legendre Daniel, Vucic Bénédicte, Hougardy Vincent, Girboux Anne-Lise, Henrioul Christophe, Van Haute Julien, Soumillion Patrice, Fastrez Jacques
Abstract excerpt
A large number of different proteins or protein domains have been investigated as possible scaffolds to engineer antibody-like molecules. We have previously shown that the TEM-1 beta-lactamase can accommodate insertions of random sequences in two loops surrounding its active site without compromising its activity. From the libraries that were generated, active enzymes binding with high affinities to monoclonal...
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