Article
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Nature structural & molecular biology - 1 Apr 2011
Rizk Shahir S, Paduch Marcin, Heithaus John H, Duguid Erica M, Sandstrom Andrew, Kossiakoff Anthony A
Abstract excerpt
We describe a phage display methodology for engineering synthetic antigen binders (sABs) that recognize either the apo or the ligand-bound conformation of maltose-binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP act as positive allosteric effectors by substantially increasing the affinity for maltose. A crystal structure of a sAB bound to the closed form of MBP reveals the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.