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Article

Promiscuous Protein Binding as a Function of Protein Stability.

Structure (London, England : 1993) - 5 Dec 2017

Cohen-Khait Ruth, Dym Orly, Hamer-Rogotner Shelly, Schreiber Gideon

Abstract excerpt

Proteins have evolved to balance efficient binding of desired partners with rejection of unwanted interactions. To investigate the evolution of protein-protein interactions, we selected a random library of pre-stabilized TEM1 β-lactamase against wild-type TEM1 using yeast surface display. Three mutations were sufficient to achieve micromolar affinity binding between the two. The X-ray structure emphasized that...

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Identifiers

PMID: 29211984
DOI: 10.1016/j.str.2017.11.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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