Mutations affecting transcription pausing in the Bacillus subtilis pyr operon.

Abstract excerpt

Pausing during transcription of the Bacillus subtilis pyr operon was proposed to play a role in its regulation by attenuation. Substitution mutations in the B. subtilis pyr DNA specifying the 3'-terminal nucleotides of the previously identified transcription pause sites substantially reduced pausing at these sites in vitro. This result confirms the general utility of this mutagenic strategy for studying...

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