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Article

A high-throughput assay for DNA deaminases.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Wang Meng, Rada Cristina, Neuberger Michael S

Abstract excerpt

Most members of the AID/APOBEC family of polynucleotide deaminases can catalyse the deamination of cytosine to uracil in DNA. They thereby function as active DNA mutators. Here, we describe how bacterial papillation assays can be adapted to monitor the mutator activity of AID/APOBEC proteins and show how such papillation assays can be used as a high-throughput screen to identify AID variants with increased...

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Source

Identifiers

PMID: 21370049
DOI: 10.1007/978-1-61779-018-8_11

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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