Article
Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein.
Journal of structural biology - 1 May 2011
Royant Antoine, Noirclerc-Savoye Marjolaine
Abstract excerpt
Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency. EGFP is extensively used in cell biology and biochemistry as a colocalization or expression reporter. Surprisingly, the structure of this very popular protein has not been determined yet. We report here its...
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