Article
Photophysics of EGFP (E222H) Mutant, with Comparisons to Model Chromophores: Excited State pK's, Progressions, Quenching and Exciton Interaction.
Journal of fluorescence - 1 May 2017
Kirk William, Allen Thomas, Atanasova Elena, Wessels William, Yao Janet, Prendergast Franklyn
Abstract excerpt
A novel version of the well-known and commercially successful Green Fluorescent Protein (GFP) variant known as EGFP, with an introduced E222H mutation, was produced in this laboratory. Given the current state of hypotheses about the role of glutamate 222, and the observed dominance of the phenolate absorption with an E222H variant observed from earlier study, the new mutant was considered a natural choice to...
Topics
- Electrons
- Green Fluorescent Proteins
- Models, Molecular
- Mutation
- Physical Phenomena
- Quantum Theory
- Spectrometry, Fluorescence