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Article

Sensitive detection of KRAS mutations in archived formalin-fixed paraffin-embedded tissue using mutant-enriched PCR and reverse-hybridization.

The Journal of molecular diagnostics : JMD - 1 Nov 2009

Ausch Christoph, Buxhofer-Ausch Veronika, Oberkanins Christian, Holzer Barbara, Minai-Pour Michael, Jahn Stephan, Dandachi Nadia, Zeillinger Robert, Kriegshäuser Gernot

Abstract excerpt

Recently, evidence has emerged indicating that assessment of KRAS mutations before anti-epidermal growth factor receptor therapy improves outcome in patients with metastatic colorectal cancer (CRC). We report here a novel reverse-hybridization (RH) assay to screen for KRAS mutations in formalin-fixed paraffin-embedded colorectal tissue samples. We combined mutant-enriched PCR based on peptide nucleic acid...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 19797612
DOI: 10.2353/jmoldx.2009.090022
PMCID: PMC2765748

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Sensitive detection of KRAS mutations in archived formalin-fixed paraffin-embedded tissue using mutant-enriched PCR and reverse-hybridization.PMID 19797612Read ↗

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