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Article

Duplex reverse-hybridization assay for the simultaneous detection of KRAS/BRAF mutations in FFPE-extracted genomic DNA from colorectal cancer specimens.

Disease markers - 1 Jan 2013

Buxhofer-Ausch Veronika, Ausch Christoph, Zeillinger Robert, Oberkanins Christian, Dandachi Nadia, Reiner-Concin Angelika, Kriegshäuser Gernot

Abstract excerpt

We report the performance evaluation of a non-quantitative reverse-hybridization assay (KRAS-BRAF StripAssay) designed for the simultaneous detection of 10 mutations in codons 12 and 13 of the KRAS gene and BRAF mutation V600E. Dilution experiments using DNA from tumor cell lines or from formalin-fixed paraffin-embedded (FFPE) colorectal cancer (CRC) tissue were performed to assess assay sensitivity. Using 50 ng...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 23324583
DOI: 10.3233/DMA-120960
PMCID: PMC3810114

Genes

BRAF
KRAS

Variants

Provenance

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