Article
Crystal structure of the TSH receptor (TSHR) bound to a blocking-type TSHR autoantibody.
Journal of molecular endocrinology - 1 Apr 2011
Sanders Paul, Young Stuart, Sanders Jane, Kabelis Katarzyna, Baker Stuart, Sullivan Andrew, Evans Michele, Clark Jill, Wilmot Jane, Hu Xiaoling, Roberts Emma, Powell Michael, Núñez Miguel Ricardo, Furmaniak Jadwiga, Rees Smith Bernard
Abstract excerpt
A complex of the TSH receptor extracellular domain (amino acids 22-260; TSHR260) bound to a blocking-type human monoclonal autoantibody (K1-70) was purified, crystallised and the structure solved at 1.9 Å resolution. K1-70 Fab binds to the concave surface of the TSHR leucine-rich domain (LRD) forming a large interface (2565 Å(2)) with an extensive network of ionic, polar and hydrophobic interactions. Mutation of...
Topics
- Amino Acids
- Animals
- Antigen-Antibody Complex
- Autoantibodies
- Autoimmune Diseases
- CHO Cells
- Cricetinae
- Cricetulus
- Crystallization
- Female
- Humans
- Hydrophobic and Hydrophilic Interactions
- Models, Molecular