Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification.

doi:10.1007/s10529-011-0518-7

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Article

Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification.

Biotechnology letters - 1 May 2011

Luhe Annette Lin, Tan Lily, Wu Jinchuan, Zhao Hua

Abstract excerpt

Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l(-1) compared to...

Topics

Antifungal Agents
Drug Resistance, Fungal
Ethanol
Genome, Fungal
Glucose
Microbial Viability
Mutation
Polymerase Chain Reaction
Recombination, Genetic
Saccharomyces cerevisiae
Transformation, Genetic

Source

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Identifiers

PMID: 21246255
DOI: 10.1007/s10529-011-0518-7

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