Article
Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification.
Biotechnology letters - 1 May 2011
Luhe Annette Lin, Tan Lily, Wu Jinchuan, Zhao Hua
Abstract excerpt
Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l(-1) compared to...
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