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Article

High-pressure EPR reveals conformational equilibria and volumetric properties of spin-labeled proteins.

Proceedings of the National Academy of Sciences of the United States of America - 25 Jan 2011

McCoy John, Hubbell Wayne L

Abstract excerpt

Identifying equilibrium conformational exchange and characterizing conformational substates is essential for elucidating mechanisms of function in proteins. Site-directed spin labeling has previously been employed to detect conformational changes triggered by some event, but verifying conformational exchange at equilibrium is more challenging. Conformational exchange (microsecond-millisecond) is slow on the EPR...

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Identifiers

PMID: 21205903
DOI: 10.1073/pnas.1017877108
PMCID: PMC3029758

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Source receipt: pubmed, retrieved 7 Sept 2026.

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