Article
Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): a study of doubly-spin-labeled T4 lysozyme.
Journal of magnetic resonance (San Diego, Calif. : 1997) - 1 Mar 2012
Georgieva Elka R, Roy Aritro S, Grigoryants Vladimir M, Borbat Petr P, Earle Keith A, Scholes Charles P, Freed Jack H
Abstract excerpt
Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the conformation of biomacromolecule and/or spin-label. We studied in detail the effect of these experimental variables on the distance distributions obtained by DEER from a series of doubly...
Topics
- Ampicillin Resistance
- Bacteriophage T4
- Electron Spin Resonance Spectroscopy
- Freezing
- Glycerol
- Models, Molecular
- Muramidase
- Mutation
- Plasmids
- Protein Conformation
- Spin Labels