Article
Pressure-jump-induced kinetics reveals a hydration dependent folding/unfolding mechanism of ribonuclease A.
Biophysical journal - 15 Sept 2006
Font J, Torrent J, Ribó M, Laurents D V, Balny C, Vilanova M, Lange R
Abstract excerpt
Pressure-jump (p-jump)-induced relaxation kinetics was used to explore the energy landscape of protein folding/unfolding of Y115W, a fluorescent variant of ribonuclease A. Pressure-jumps of 40 MPa amplitude (5 ms dead-time) were conducted both to higher (unfolding) and to lower (folding) pressure, in the range from 100 to 500 MPa, between 30 and 50 degrees C. Significant deviations from the expected symmetrical...
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