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Article

Using CEST NMR to discover previously unobserved states on the free energy surface of proteins: Application to the L99A cavity mutant of T4 lysozyme.

The Journal of biological chemistry - 1 Jan 2026

Tiwari Ved Prakash, Khandave Nihar Pradeep, Hansen D Flemming, Bouvignies Guillaume, Kay Lewis E, Vallurupalli Pramodh

Abstract excerpt

Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments establish that at room temperature, the L99A cavity mutant of T4 lysozyme (L99A T4L) interconverts between two compact folded conformations on the millisecond timescale. These include the native state in which the sidechain of Phe114 is exposed to solvent (E state) and a near-native minor state in which the aromatic moiety of Phe114 is buried in...

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Identifiers

PMID: 41317969
DOI: 10.1016/j.jbc.2025.110989
PMCID: PMC12805379

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