Article
Membrane topology of NAADP-sensitive two-pore channels and their regulation by N-linked glycosylation.
The Journal of biological chemistry - 18 Mar 2011
Hooper Robert, Churamani Dev, Brailoiu Eugen, Taylor Colin W, Patel Sandip
Abstract excerpt
Two-pore channels (TPCs) localize to the endolysosomal system and have recently emerged as targets for the Ca(2+)-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). However, their membrane topology is unknown. Using fluorescence protease protection assays, we show that human TPC1 and TPC2 possess cytosolic N and C termini and therefore an even number of transmembrane regions....
Topics
- Calcium Signaling
- Cell Line, Tumor
- Female
- Glycosylation
- HEK293 Cells
- Humans
- Ion Channel Gating
- Mutation
- NADP
- Protein Modification, Translational
- Protein Structure, Secondary